The nuclear localized RIN13 induces cell death through interacting with ARF1.
Identifieur interne : 000019 ( Main/Exploration ); précédent : 000018; suivant : 000020The nuclear localized RIN13 induces cell death through interacting with ARF1.
Auteurs : Xiaoxiao Liu [République populaire de Chine] ; Hui Liu [République populaire de Chine] ; Wen-Cheng Liu [République populaire de Chine] ; Zhiyong Gao [République populaire de Chine]Source :
- Biochemical and biophysical research communications [ 1090-2104 ] ; 2020.
Abstract
Resistance to Pseudomonas syringae pv. Maculicola 1 (RPM1) is a crucial immune receptor conferring plant enhanced resistance to pathogenic bacteria. RPM1-interacting protein 13 (RIN13) enhances RPM1-mediated disease resistance through interacting with the central domain of RPM1 in Arabidopsis, while the underlying mechanism remains elusive. Here, we report the subcellular localization and function of RIN13 using the Nicotiana benthamiana (N. benthamiana) transient expression system. Our results showed that RIN13 is exclusively localized in the nucleus, and RIN13 (231-300) fragment is responsible for its nuclear localization. Transient expression of RIN13 in N. benthamiana leaves can accelerate leaf senescence and cell death, and affect the activities of ROS-scavenging enzymes, and the C-terminus of RIN13 is crucial for its function. Furthermore, we identified a RIN13-interacting protein, Auxin Response Factor 1 (ARF1), and found that similar to RIN13, ARF1 can also promote leaf senescence and cell death. In addition, expression of RIN13 in N. benthamiana leaves can facilitate the translocation of ARF1 into the nucleus. Collectively, our study revealed a possible mechanism of RIN13 in accelerating leaf senescence and cell death by changing the subcellular localization of ARF1.
DOI: 10.1016/j.bbrc.2020.04.082
PubMed: 32446355
Affiliations:
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Electronic address: liuwencheng@henu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Cotton Biology, State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004</wicri:regionArea><wicri:noRegion>475004</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Zhiyong" sort="Gao, Zhiyong" uniqKey="Gao Z" first="Zhiyong" last="Gao">Zhiyong Gao</name><affiliation wicri:level="4"><nlm:affiliation>State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, 430072, China. Electronic address: zygao@whu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, 430072</wicri:regionArea><orgName type="university">Université de Wuhan</orgName><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="eISSN">1090-2104</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Resistance to Pseudomonas syringae pv. Maculicola 1 (RPM1) is a crucial immune receptor conferring plant enhanced resistance to pathogenic bacteria. RPM1-interacting protein 13 (RIN13) enhances RPM1-mediated disease resistance through interacting with the central domain of RPM1 in Arabidopsis, while the underlying mechanism remains elusive. Here, we report the subcellular localization and function of RIN13 using the Nicotiana benthamiana (N. benthamiana) transient expression system. Our results showed that RIN13 is exclusively localized in the nucleus, and RIN13 (231-300) fragment is responsible for its nuclear localization. Transient expression of RIN13 in N. benthamiana leaves can accelerate leaf senescence and cell death, and affect the activities of ROS-scavenging enzymes, and the C-terminus of RIN13 is crucial for its function. Furthermore, we identified a RIN13-interacting protein, Auxin Response Factor 1 (ARF1), and found that similar to RIN13, ARF1 can also promote leaf senescence and cell death. In addition, expression of RIN13 in N. benthamiana leaves can facilitate the translocation of ARF1 into the nucleus. Collectively, our study revealed a possible mechanism of RIN13 in accelerating leaf senescence and cell death by changing the subcellular localization of ARF1.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">32446355</PMID><DateRevised><Year>2020</Year><Month>05</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2104</ISSN><JournalIssue CitedMedium="Internet"><Volume>527</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>Jun</Month><Day>18</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem Biophys Res Commun</ISOAbbreviation></Journal><ArticleTitle>The nuclear localized RIN13 induces cell death through interacting with ARF1.</ArticleTitle><Pagination><MedlinePgn>124-130</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0006-291X(20)30808-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bbrc.2020.04.082</ELocationID><Abstract><AbstractText>Resistance to Pseudomonas syringae pv. Maculicola 1 (RPM1) is a crucial immune receptor conferring plant enhanced resistance to pathogenic bacteria. RPM1-interacting protein 13 (RIN13) enhances RPM1-mediated disease resistance through interacting with the central domain of RPM1 in Arabidopsis, while the underlying mechanism remains elusive. Here, we report the subcellular localization and function of RIN13 using the Nicotiana benthamiana (N. benthamiana) transient expression system. Our results showed that RIN13 is exclusively localized in the nucleus, and RIN13 (231-300) fragment is responsible for its nuclear localization. Transient expression of RIN13 in N. benthamiana leaves can accelerate leaf senescence and cell death, and affect the activities of ROS-scavenging enzymes, and the C-terminus of RIN13 is crucial for its function. Furthermore, we identified a RIN13-interacting protein, Auxin Response Factor 1 (ARF1), and found that similar to RIN13, ARF1 can also promote leaf senescence and cell death. In addition, expression of RIN13 in N. benthamiana leaves can facilitate the translocation of ARF1 into the nucleus. Collectively, our study revealed a possible mechanism of RIN13 in accelerating leaf senescence and cell death by changing the subcellular localization of ARF1.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xiaoxiao</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, 430072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Hui</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, 430072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Wen-Cheng</ForeName><Initials>WC</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Cotton Biology, State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, China. Electronic address: liuwencheng@henu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Zhiyong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, 430072, China. Electronic address: zygao@whu.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ARF1</Keyword><Keyword MajorTopicYN="N">Cell death</Keyword><Keyword MajorTopicYN="N">Plant defense</Keyword><Keyword MajorTopicYN="N">RIN13</Keyword><Keyword MajorTopicYN="N">ROS</Keyword><Keyword MajorTopicYN="N">RPM1</Keyword></KeywordList><CoiStatement>Declaration of competing interest No potential conflict of interest is disclosed.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>04</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32446355</ArticleId><ArticleId IdType="pii">S0006-291X(20)30808-1</ArticleId><ArticleId IdType="doi">10.1016/j.bbrc.2020.04.082</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Liu, Xiaoxiao" sort="Liu, Xiaoxiao" uniqKey="Liu X" first="Xiaoxiao" last="Liu">Xiaoxiao Liu</name></region><name sortKey="Gao, Zhiyong" sort="Gao, Zhiyong" uniqKey="Gao Z" first="Zhiyong" last="Gao">Zhiyong Gao</name><name sortKey="Liu, Hui" sort="Liu, Hui" uniqKey="Liu H" first="Hui" last="Liu">Hui Liu</name><name sortKey="Liu, Wen Cheng" sort="Liu, Wen Cheng" uniqKey="Liu W" first="Wen-Cheng" last="Liu">Wen-Cheng Liu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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